Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensus PCR HPV-DNA, and In Situ Hybridization

Pannone, Giuseppe; Rodolico, Vito; Santoro, Angela; Lo Muzio, Lorenzo; Franco, Renato; Botti, Gerardo; Aquino, Gabriella; Pedicillo, Maria Carmela; Cagiano, Simona; Campisi, Giuseppina; Rubini, Corrado; Papagerakis, Silvana; De Rosa, Gaetano; Tornesello, Maria Lina; Buonaguro, Franco M; Staibano, Stefania; Bufo, Pantaleo

doi:10.1186/1750-9378-7-4

Table 1 Interaction between HPV virus detection as evaluated by molecular methods and in situ hybridization signals.

From: Evaluation of a combined triple method to detect causative HPV in oral and oropharyngeal squamous cell carcinomas: p16 Immunohistochemistry, Consensus PCR HPV-DNA, and In Situ Hybridization

Case	Year	Age-Sex	Italian Region	Site	HistologicalGrade	pTNM stage	In situ hybridization	Consensus PCR based method
OPSCC-1	2004	69 F	Puglia	Tonsil	G1	Biopsy	Negative	negative
OPSCC-2	2006	79 F	Puglia	Tonsil	G3	Biopsy	Negative	negative
OPSCC-3	2007	55 M	Puglia	Tonsil	G2	Biopsy	Negative	negative
OPSCC-4	ND	51 F	Puglia	Tonsil	G2	Biopsy	Negative	negative
OPSCC-5	2008	47 M	Puglia	Tonsil	G2	Biopsy	Negative	negative
OPSCC-6	2008	63 M	Puglia	Tonsil	G3	Biopsy	Negative	negative
OPSCC-7	2009	46 M	Puglia	Tonsil positive Uvula	G2	Biopsy	Negative	negative
OPSCC-8	2006	54 M	Puglia	Tonsil	G2	Biopsy	HR-HPV: nuclear clusters	negative
OPSCC-9	2008	45 M	Campania	Tonsil	G2	pT2NxM x	HR-HPV: integration positive clusters	HPV16
OPSCC-10	2007	60 F	Campania	Tonsil	G3	pT1NxM x	negative	HPV16
OPSCC-11	2007	62 M	Campania	Tonsil	ND	pT2NxM x	negative	HPV16
OPSCC-12	2007	51 M	Campania	Tonsil	G3	pT1NxMx	negative	negative
OPSCC-13	2008	67 M	Campania	Tonsil	G3	pT1NxMx	HR-HPV: clusters	HPV16
OPSCC-14	2006	55 F	Campania	Tonsil	G3	pTxNxMx	negative	negative
OPSCC-15	2009	69 F	Campania	Tonsil	G3	pT2NxMx	HR-HPV: focal clusters	HPV16
OPSCC-16	2008	79 F	Campania	Tonsil	G2	pT4aNxMx	negative	negative
OPSCC-17	2008	87 M	Campania	Tonsil	G3	pT1NxMx	HR-HPV: focal clusters	negative
OPSCC-18	2009	62 M	Campania	Tonsil	G3	pT4aNxMx	ND	HPV16
OPSCC-19	2009	66 M	Campania	Lingual tonsil - vallecula	G3	pT2N2bM0	negative	ND
OPSCC-20	2007	61 M	Sicilia	Tonsil	G3	pT2N0Mx	HR-HPV integrative	negative
OPSCC-21	2009	42 M	Sicilia	Tonsil	G2	pTxNxMx	negative	negative
OPSCC-22	2011	60 M	Sicilia	Tonsil	G3	pT2N0Mx	HR-HPV: focal nuclear clusters	ND
OSCC-1	1999	75 F	Marche	Tongue	G2	pT1N0M0	cytoplasmic signals	HPV31/44
OSCC-2	2000	59 M	Marche	Oral cavity (WRA)	G3	pT2N2bM0	negative (not available probes)	HPV 53
OSCC-3	2001	48 M	Marche	Tongue base (WRA)	G1	pT1N0M0	negative (not available probes)	HPV 70
OSCC-4	2002	63 M	Marche	Tongue	G3	pT2N1M0	ND	HPV 6
OSCC-5	1999	74 M	Marche	Oral cavity (WRA)	G3	pT2N0M0	HR-HPV: integration positive focal clusters	HPV 16
OSCC-6	2009	72 M	Sicilia	Oral cavity	G1	pT1N0M0	HR-HPV: faint focal nuclear clusters	HPV16/56
OSCC-7	2009	49 M	Sicilia	Tongue	G2	pT2N1Mx	negative	negative
OSCC-8	2010	41 M	Sicilia	Tongue	G2	pT1N0Mx	negative	negative
OSCC-9	2008	37 M	Sicilia	Hard palate	G1	pT2N0Mx	negative	negative
OSCC-10	2009	72 M	Sicilia	Oral cavity	G1	pT1N0M0	negative	HPV16/56
OSCC-11	2010	74M	Puglia	Tongue	G1	pT2N0M0	negative	ND
TMA OSCC (53 cases)	1997-2008	20 F/33 M	Campania	Oral cavity- multiple sites	All G available	All TNM available	negative	negative

The results are compared to clinic-pathologic parameters and demographic data
TMA: tissue microarray; ND: non determined data; F: female; M: male; WRA: Waldeyer Ring Area

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ISSN: 1750-9378

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