Table 4 Oligonucleotides used
Primers and probes | Orientation | Sequence (5’ 3’) | References |
|---|---|---|---|
HHV-6 detection | |||
Taq1 | + | GACAATCACATGCCTGGATAATG | [21] |
Taq 2 | - | TGTAAGCGTGTGGTAATGGACTAA | |
H6S probe | FAM-AGCAGCTGGCGAAAAGTGCTGTGC-TAMRA | ||
Human albumin gene | |||
Alb-forward | + | GCTGTCATCTCTTGTGGGCTGT | [22] |
Alb-reverse | - | ACTCATGGGAGCTGCTGGTTC | |
Alb-probe | FAM-CCTGTCATGCCCACACAAATCTCTCC-TAMRA | ||
HHV-6 (A and B) | |||
HHV-6A | - | TGGTAATGGACTAATTGTGTGTTGTTTTA | [23] |
HHV-6B | - | TGGTAATGGACTAAGTGTGCGTTATTTTC | |