The viral transactivator HBx protein exhibits a high potential for regulation via phosphorylation through an evolutionarily conserved mechanism

Table 2 Phosphorylation prediction on HBx

Conserved residues	NetPhos 2.0	DISPHOS 1.3	PPRED	Phos3D	NetSurfP 1.1	NetPhosK 1.0	KinasePhos	PPSP	GPS 2.1	YinOYang 1.2
Serines
S25	0.961	0.762	―	+	E	CKI	―	―	TKL MLK ILK	―
S41	0.983	0.930	+	+	E	P38MAPK GSK3 cdk5	cdc2 MAPK	KIS	CMGC MAPK ERK	+
S54	0.652	0.672	―	―	E	CKI PKC	―	MAPKKK	―	―
S75	0.984	0.672	―	―	B	―	―	―	AGC PKC CAMK	―
S153	0.005	0.187	―	―	E	―	―	―	AGC GRK TKL	―
Threonines
T74	0.147	0.115	―	―	B	PKC	―	PKR	―	―
T81	0.947	0.823	+	+	E	―	PKA	MAPKKK	CAMK DAPK LKB	+
T97	0.017	0.085	―	―	E	PKG	PKA	ILK	TLK MLK ILK	―
T106	0.524	0.113	―	―	E	―	―	―	AGC SGK CAMKL	―
Tyrosines
Y111	0.031	0.062	―	―	B	―	―	―	TK PDFGR Eph	―

Netphos: threshold > 0.5; DISPHOS: threshold >0.5; PPRED, Phos3D: parameters as default; NetSufP: E: exposed surface;
B: buried surface; O-β-GlcNAc: threshold as default.

ISSN: 1750-9378