The viral transactivator HBx protein exhibits a high potential for regulation via phosphorylation through an evolutionarily conserved mechanism

Table 1 Bioinformatics tools used for prediction

Name	Technique	Residues	Reference	Website
Phosphorylation
NetPhos 2.0	ANN	9-33	[51]	http://cbs.dtu.dk/services/NetPhos
DISPHOS 1.3	LR	25	[52]	http://dabi.temple.edu/disphos
PPRED	PSSM, SVM	7-15	[54]	http://ashiskb.info/reserach/ppred
Phos3D	SVM	13	[53]	http://phos3d.mpimp-golm.mpg.de
Kinases
NetPhosK 1.0	ANN	9-33	[55]	http://cbs.dtu.dk/services/NetPhosK
KinasePhos	SVM	9	[56]	http://kinasephos2.mbc.nctu.edu.tw
PPSP	BP	9	[57]	http://ppsp.biocuckoo.org
GPS 2.1	PSSM, GA	3-31	[58]	http://gps.biocuckoo.org

Techniques: ANN: Artificial neural network; LR: Logistic regression; SVM: Support vector machine;
PSSM: Position-specific scoring matrix; BP: Bayesian probability; GA: Genetic algorithm.

ISSN: 1750-9378