Figure 2

Predicted O-glycosylation and Yin Yang sites on the HBx protein. The O-β-GlcNAc modification potential of each Ser, and Thr residues is shown by green vertical lines, and the light blue horizontal wavy line indicates the threshold for modification potential. The Yin Yang sites that were positively predicted are shown by red asterisks at the top. The profile was obtained with server YinOYang 1.2, using the HBx sequence of our HBV isolate 4.5 as a reference. The numbers at the top of lines of O-glycosylation potential indicate the positions of conserved HBx Ser, Thr or Tyr residues. As shown, Ser25 represents a FN Yin Yang site.