Figure 5

Percentage of splenic CD11b+Gr-1+cells in mock treated and HV-68 infected mice with metastatic breast cancer. Groups of mice were mock treated or HV-68 infected. Six months post-infection, mice were injected with syngeneic 4 T1 mammary tumor cells in the mammary fat pad. At day 44 following tumor cell transplantation, groups of mice were euthanized and tissues were isolated. Bone marrow cells and splenocytes were isolated and labeled with fluorchrome-conjugated anti-CD11b and anti-Gr-1 antibodies. FACS analysis of one representative animal per group shows the percentage of CD11b+Gr-1+cells (upper right quadrant) from the bone marrow (Panel A) or spleen (Panel C) of a mock-treated mouse, or from the bone marrow (Panel B) or spleen (Panel D) of an HV-68 infected mouse that have 4 T1 breast cancer metastases