Figure 4

ELISA quantification of S100A8 and S100A9 in sera of mock treated or HV-68 infected mice with metastatic breast cancer. Groups of mice were mock treated or HV-68 infected. Six months post-infection, mice were injected with syngeneic 4 T1 mammary tumor cells into the mammary fat pad. At day 44 following tumor cell transplantation, groups of mice were euthanized and sera isolated from individual animals. Each serum sample was assayed for the presence of S100A8 and S100A9 using an ELISA capable of recognizing either protein. Results are presented as means (N = 6) ± standard errors