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Figure 3 | Infectious Agents and Cancer

Figure 3

From: An expanded myeloid derived suppressor cell population does not play a role in gammaherpesvirus-exacerbated breast cancer metastases

Figure 3

Extracellular levels of S100A8 and S100A9 proteins do not increase in macrophages or dendritic cells with increasing doses of HV-68. Bone marrow-derived macrophages (Panel A) or dendritic cells (Panel B) were incubated for 24 hrs with increasing amounts of HV-68 virus. Medium was mixed with 2×SDS sample buffer and protein was electrophoresed on SDS 15% polyacrylamide gels. Protein transferred to Immobilon P was probed with anti-S100A8 and S100A9 antibodies. S100A8 and S100A9 protein levels in the medium were unchanged as a result of increased amounts of HV-68 virus. Similarly, intracellular S100A8 and S100A9 levels were unchanged (data not shown)

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