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Figure 2 | Infectious Agents and Cancer

Figure 2

From: An expanded myeloid derived suppressor cell population does not play a role in gammaherpesvirus-exacerbated breast cancer metastases

Figure 2

Extracellular levels of S100A8 and S100A9 proteins do not increase after a time course of incubation of macrophages or dendritic cells with HV-68. Bone marrow-derived macrophages (Panel A) or dendritic cells (Panel B) were incubated with HV-68 virus (MOI of 1:1) from zero to 48 hrs or with LPS (1 ng/ml). Medium was mixed with 2×SDS sample buffer, boiled and proteins electrophoresed on 15% polyacrylamide gels and protein transferred to Immobilon P probed with anti-S100A8 and anti-S100 A9 antibodies. S100A8 and S100A9 protein levels were either unchanged or reduced during the time course of the incubation. Similarly, cell lysates did not show decreased levels of intracellular S100A8 or S100A9 (data not shown)

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