Figure 1

HV-68 virus does not induce increased S100A8 and S100A9 mRNA expression in macrophages or dendritic cells in vitro. Bone marrow-derived macrophages (Top panels A and B) or dendritic cells (Bottom panels C and D) were incubated with HV-68 virus for various lengths of time (Panels A and C-time course) or different multiplicities of infection (Panels B and D-dose-response). mRNA was isolated, treated with DNase to remove genomic DNA and used to synthesize cDNA. The time course of the viral induction of S100A8 and S100A9 mRNA was assessed by semiquantitative PCR. The viral ORF65 PCR product is presented as a proxy for viral gene expression and the PCR product from the constitutively expressed housekeeping gene GAPDH is shown to demonstrate similar amounts of cDNA in each sample. The results are presented as amplified products electrophoresed on ethidium bromide-stained agarose gels. DNA sizes in base pairs are presented to the left of the DNA standard