Figure 4

H & E staining to visualize metastatic lung lesions in untreated, mock and HV-68 infected mice. At day 44 following tumor cell transplantation, groups of mice (N = 8–10) were euthanized. Lungs were taken from each animal, fixed in formalin, and paraffin embedded for H & E staining. Left panels ( A, C, E, G, and I) show 20X magnification and right panels ( B, D, F, H, and J) show 40X magnifications of the same micrographs. Panels A and B show representative microscopic lung sections from an untreated mouse. Panels C, D, E and F show representative microscopic lung sections from two mock treated mice that were transplanted with 4 T1 tumor cells. Panels G, H, I and J show representative microscopic lung sections from two HV-68 infected mice transplanted with 4 T1 tumor cells. Circles show the location of clearly discernable metastatic lesions in HV-68 infected mice.