Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma

Table 4 Primers and specific probes used for HPV detection and typing

Primers			Sequence 5'→3'
Primers for β-globin amplification	PC 04		CAA CTT CAT CCA CGT TCA CC
	GH 20		GAA GAG CCA AGG ACA GGT AC
BZLF1 Primers for EBV amplification	ZES		GCC ACC TTT GCT ATC TTT GC
	ZEAS		AGG CGT GGT TTC AAT AAC GG
Primers for HPV amplification	MY 09		CGT CCM ARR GGA WAC TGA TC
	MY 11		GCM CAG GGW CAT AAY AAT GG
	HPV 16	MY14	CAT ACA CCT CCA GCA CCT AA
	HPV 18	WD74	GGA TGC TGC ACC GGC TGA
	HPV 31	WD126	CAA AAG CCC AAG GAA GAT C
Specific probes for HPV typing	HPV 33	MY16	CAC ACA AGT AAC TAG TGA CAG
	HPV 35	MY115	CTG CTG TGT CTT CTA GTG ACA G
	HPV 45	MY70	TAG TGG ACA CTA CCC GCA G
	HPV 59	MY123	GCC AGT TAA ACA GGA CCC

ISSN: 1750-9378