Figure 1

MPCR electropherogram in multiple infection. An autoscaled electropherogram of a single CIN 3 sample contained HPV E6/E7 types 16, 31, 45, 59 and 66, which are resolved in three panels, each representing a separate color channel (a red size marker channel is not shown). The X-axis indicates product size in nucleotides and the Y-axis shows fluorescence intensity in arbitrary units. The upper panel contains a single blue peak at 169 nt, corresponding to HPV59. In the middle panel, green peaks at 121, 210 and 268 nt confirm HPV31, HPV16 and the β-globin control, respectively. The bottom panel's black peak at 106 nt indicates HPV45 and the second peak at 121 nt matches HPV66. In the routine electropherogram with composite channels, peaks for types 31 and 66 overlap but are easily identified by color.