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Table 1 Ct values in direct and nested qPCR E6-2 amplicon mixtures with a variable number of pHV101 copies

From: Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

pHV101 copies Ct ΔCt
  Direct qPCR Nested qPCR  
2.5 × 102 --- 25.02 ---
2.5 × 103 29.00 21.62 7.40
2.5 × 104 25.90 17.71 8.10
2.5 × 105 22.10 14.35 7.75
2.5 × 106 18.70 10.80 7.90