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Table 1 Ct values in direct and nested qPCR E6-2 amplicon mixtures with a variable number of pHV101 copies

From: Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR

pHV101 copies

Ct

ΔCt

 

Direct qPCR

Nested qPCR

 

2.5 × 102

---

25.02

---

2.5 × 103

29.00

21.62

7.40

2.5 × 104

25.90

17.71

8.10

2.5 × 105

22.10

14.35

7.75

2.5 × 106

18.70

10.80

7.90

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Infectious Agents and Cancer

ISSN: 1750-9378

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