Figure 1
Family of E6-2 amplification curves obtained by direct and nested qPCR. RFU, relative fluorescence units. Threshold (thick horizontal line): 50 RFU. (A) Family of curves obtained in direct qPCR mixtures with pHV101 dilutions and primers for E6-2. Curve 1, 2.5 × 106 pHV101 copies. Curve 2, 2.5 × 105 copies. Curve 3, 2.5 × 104 copies. Curve 4, 2.5 × 103 copies. Curve 5, Blank (without DNA). (B) Family of curves obtained by nested qPCR with E6-2 primers and 1/50 volume of mixtures preamplified 15 cycles with pHV101 and E6-1 primers. Curve 1, 2.5 × 106 pHV101 copies. Curve 2, 2.5 × 105 copies. Curve 3, 2.5 × 104 copies. Curve 4, 2.5 × 103 copies. Curve 5, 2.5 × 102 copies. Curve 6, blank (without DNA).