Table 1 Bisulfite Converted Gene-Specific PCR Primer Sequences
From: Epstein-Barr virus-specific methylation of human genes in gastric cancer cells
ICAM1 | Forward | 5'-TGG GGG TTG TGG TTT TAG TT-3' |
|---|---|---|
| Â | Reverse | 5'-CTC CCT CCA CTA AAA AC-3' |
| Â | Amplicon size | 412 bp |
CDKN2A (p16) | Forward | 5'-AGA TGT TTT GTG GTT GTT GTG A-3' |
| Â | Reverse | 5'-CAA AAA TCT TCC ATT CTT CAA AC-3' |
| Â | Amplicon size | 418 bp |
ID4 | Forward | 5'-TTT TTT GGG TAT ATA TTA GTT TGG-3' |
| Â | Reverse | 5'-TAT CCT AAT CAC TCC CTT C-3' |
| Â | Amplicon size | 477 bp |
COX2 | Forward | 5'-TAT GTG TTG TAT ATA GAG TAG A-3' |
| Â | Reverse | 5'-AAA AAA TAA TCC CCA CTC TC -3' |
| Â | Amplicon size | 399 bp |
TFF1 | Forward | 5'-TTA GGT TGG AGT GTA GTA GG-3' |
| Â | Reverse | 5'-CCT ACT CAT ATC TAA AAA ACC C-3' |
| Â | Amplicon size | 489 bp |
C8orf4 control | Forward | 5'-GAA TTA AAA TAT AAG GAG AGT TTT-3' |
| Â | Reverse | 5'-AAC ATT ACC CAA ACA TAA AAC AA-3' |
| Â | Amplicon size | 328 bp |