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Table 3 Primers used for HCMV DNA amplification by real-time quantitative PCR

From: Analysis of colorectal cancers for human cytomegalovirus presence

primer

sequence

amplicon length

gene position

US28 S

TCCATCGGCAACTTCTTGGT

65 bp

267671 to 267690

HHV5-US28As

TCGCCGGAGCATTGAATC

 

267718 to 267735

  1. Oligonucleotide primers used for amplification of the HCMV US28 gene in real-time PCR. Primers were designed using Primer Express V2.0 software (Applied Biosystems) on the sequence of US28 gene of the AD169 laboratory strain and controlled by aligning sequence with BLAST.
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Infectious Agents and Cancer

ISSN: 1750-9378

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