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Figure 4 | Infectious Agents and Cancer

Figure 4

From: Prevalence of high-risk human papillomavirus types in Mexican women with cervical intraepithelial neoplasia and invasive carcinoma

Figure 4

Inhibition of L1-450 and E6-250 segment amplification in duplex PCR mixtures with primer sets MY 09/11 and pU 1M/2R. Mixtures contained 50 ng DNA from Patient 1 as well as primer set MY 09/11, pU 1M/2R, or both. Arrows to the left indicate positions of the bands expected. Lane 1, 100 bp ladder. Lane 2, Uniplex 1 mixture (set MY 09/11). Lane 3, Uniplex mixture 2 (set pU 1M/2R). Lane 4, Duplex mixture (sets MY 09/11 and pU 1M/2R). Lane 5, Negative control (without DNA). Major bands expected in uniplex and duplex mixtures: ~450 bp with set MY 09/11 (Lanes 2 and 4) and ~250 bp with set pU 1M/2R (Lanes 3 and 4). Note the decrease in the intensity of bands in the duplex mixture (lane 4), especially ~450 bp.

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