Figure 3

Algorithm for HR-HPV typing. The PCR-RFLP method used identifies seven HR-HPV types by the size of the restriction fragments of the E6-250 products generated by specific cuts with five enzymes. Ava II is used first because it helps recognize types 16, 18 and 33 (16 and 18 are known to be the most prevalent globally). PCR products not restricted by Ava II are incubated separately with each of the four remaining enzymes to identify the other five HR-HPV types.