Figure 4

Induction of gp120-specific IgG and IgM responses in mice immunized with gp120 or gp120/mAb complex. Sera were collected a week after each injection and tested by ELISA at a dilution of 1:100. Sera were added to gp120LAI captured on the wells and gp120-specific IgG or IgM were detected with alkaline phosphatase-conjugated rabbit Abs specific for the respective mouse IgG or IgM.