Figure 2

IFA microphotographs showing BCBL-1 cytospin cells reactivity to anti-HHV-8 LANA (pink intranuclear dots) [a] and to lytic (green diffuse cytoplasmic staining) antibodies K8.1 [b] and ORF 59 [c] 72 hrs after treatment with tetra-phobol acetate (TPA) to induce the lytic phase. KS patient's serum was added to BCBL-1 TPA treated cytospin cells producing, the lytic green diffuse cytoplasmic staining and latent white intranuclear dots. Note that majority of BCBL-1 cells reacted to latent (brown arrow) compared lytic (white arrow) antibodies in the index KS patient in [d], all sections × 400.