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Table 2 Comparison of Standard Curves from Real-time PCR Repeat Runs

From: Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens

Run

r2

Slope

Intercept

Efficiency

Pparallel

Pcoincident

HPV16-1

0.994

-3.386

35.878

97.4%

0.9640

0.8790

HPV16-2

0.997

-3.434

36.290

95.5%

  

HPV16-3

0.996

-3.450

36.423

94.9%

  

HPV16-4

0.997

-3.442

36.536

95.2%

  

HPV18-1

0.966

-2.999

37.714

115.5%

0.8753

0.1479

HPV18-2

0.990

-3.054

35.629

112.5%

  
  1. Note:
  2. Pparallel tests that the curves are parallel to one another (i.e., they have the same slope)
  3. Pcoincident tests that the curves are coincident (i.e., they have the same slope and y-intercept)
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Infectious Agents and Cancer

ISSN: 1750-9378

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