Detection and identification of enterohemorrhagic Escherichia coli O157:H7 and Vibrio cholerae O139 using oligonucleotide microarray

Table 5 Cutoff value of the probe

Target gene	Species	Average of negative bacteria and blank control signal intensity	SD	Cutoff value^a
ctxA	Vibrio cholerae O139	1783.12	64.27	1847.39
TcpA		1739.338	68.253	1807.591
LPSgt		1637.29	57.175	1694.465
stx1	Escherichia coli	1691.284	71.22	1762.504
Stx2	O157:H7	1790.385	78.473	1868.858
UidA		1802.1746	69.181	1871.3556
16S rDNA	internal control	1598.5629	24.75	1623.3129

^a cutoff value = average of negative bacteria and blank control signal intensity + 2 SD

ISSN: 1750-9378