Skip to main content

Table 2 Oligonucleotide probe sequences for identification of E. coli O157:H7 and Vibrio cholerae O139

From: Detection and identification of enterohemorrhagic Escherichia coli O157:H7 and Vibrio cholerae O139 using oligonucleotide microarray

Target gene

Sequence(5'-3')

Length (bp)

Tm(°C)a

Stx1

GTA CGT CTT TAC TGA TGA TTG ATA GTG GCA CAG GG

35

73.5

Stx2

AGT TAT TTT GCT GTG GAT ATA CGA GGG CTT GAT GT

35

69.6

uidA

TGG AAT TGA GCA GCG TTG G

19

70.1

ctxA

CAT ACA GTC CTC ATC CAG ATG AAC AAG AAG TTT CTG CTT TAG GTG

45

75.0

tcpA

CAG GAA GTG CCA ACT TAA ACC TAA CTA ATA TCA CGC ATG TTG AGA

45

76.8

LPSgt

TCG ATA AGA AGA GAT AAA GAT CTG AGT TAT CTA AAG ATA TTT G

43

71.2

Negative control

TCT TCG CCA GAG GCC TGC TAG CCT GGT TCA AGA TAC TAC C

40

68.5

Labeling control

Complimentary sequence of the reverse primer

20

70.5

  1. aTm(°C) was calculated using primer premier 5.0