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Table 2 Oligonucleotide probe sequences for identification of E. coli O157:H7 and Vibrio cholerae O139

From: Detection and identification of enterohemorrhagic Escherichia coli O157:H7 and Vibrio cholerae O139 using oligonucleotide microarray

Target gene Sequence(5'-3') Length (bp) Tm(°C)a
Stx1 GTA CGT CTT TAC TGA TGA TTG ATA GTG GCA CAG GG 35 73.5
Stx2 AGT TAT TTT GCT GTG GAT ATA CGA GGG CTT GAT GT 35 69.6
uidA TGG AAT TGA GCA GCG TTG G 19 70.1
ctxA CAT ACA GTC CTC ATC CAG ATG AAC AAG AAG TTT CTG CTT TAG GTG 45 75.0
tcpA CAG GAA GTG CCA ACT TAA ACC TAA CTA ATA TCA CGC ATG TTG AGA 45 76.8
LPSgt TCG ATA AGA AGA GAT AAA GAT CTG AGT TAT CTA AAG ATA TTT G 43 71.2
Negative control TCT TCG CCA GAG GCC TGC TAG CCT GGT TCA AGA TAC TAC C 40 68.5
Labeling control Complimentary sequence of the reverse primer 20 70.5
  1. aTm(°C) was calculated using primer premier 5.0