Figure 2

Cytofluorymetric analysis of apoptotic Raji cells. Raji cells were induced in the absence or in the presence of 3-ABA as for Fig.1. At the indicated times, cells were collected and subjected to Annexin V-FITC/PI staining as reported in the Methods. The upper part of the figure shows the dot blots of PI vs. Annexin V stain. All parameters and region settings were kept identical throughout all measurements. The table illustrates the percentages of necrotic cells (PI); secondary necrotic cells (PI/A); viable cells (V); Annexin V-positive (apoptotic) cells (A).