Figure 2

HPV Nested PCR in Clinical Samples and Reproducibility. Agarose gel showing PCR products of targeted DNAs extracted from two clinical samples in duplicate. The targeted β-globin DNA amplicon is 110 bp long, as seen clearly in lanes 25 and 26 (#1210), but is hardly visible in lanes 31 and 32 (#1211). Co-amplification of other human genomic DNA fragments and a positive nested PCR amplicon assure specimen adequacy in both samples. Molecular ruler = 100–1000 bp (far left). Lanes 25/26, 27/28, 29/30 = β-globin gene, MY09/MY11, GP5+/GP6+ PCR, respectively-sample #1210. Lanes 31/32, 33/34, 35/36 = β-globin gene, MY09/MY11, GP5+/GP6+ PCR, respectively-sample #1211