Figure 1
From: Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories
Comparative amplification of HPV-16 DNA with Taq DNA polymerase and with LoTemp™ HiFi®DNA polymerase. Lanes 1–8: HPV-16 plasmid DNA template at 85 × 101, 85 × 102, 85 × 103, 85 × 104, 85 × 105, 85 × 106, 85 × 107 and 85 × 108 copies per milliliter. NC: negative control. M: molecular marker. Arrows indicate the lower limit of detection by nested PCR (A) and by the 1st primary PCR (B) with Takara Taq DNA polymerase and LoTemp™ HiFi® DNA polymerase, respectively.