Table 1 HPV primers used in PCR amplification of the HPV 16 genes.
Primer | DNA sequence | Gene target | Nucleotide positions in NCBI noNC_001526 |
|---|---|---|---|
L1_Sfor | 5-GCCGTCGAC ATG TCTCTTTGGCTGCCTAGTAGGGCCA-3' | Late protein L1 | 5637–5663 |
L1_Srev | 5'- GCCGTCGAC GAT TTGTAGTAAAAATTTGCGTCC-3' | Late protein L1 | 7027–7050 |
L2_Bfor | 5'-GCGCGGATCC ATG cgacacaaacgttctgcaa -3' | Late protein L2 | 4235–4256 |
L2_Hrev | 5'-GCGCAAGCTT CTA GGCAGCCAAAGAGACATC -3' | Late protein L2 | 5636–5656 |
E6_Bfor | 5'-GCGCGGATCC ATG CACCAAAAGAGAACTGC3' | Early protein E6 | 83–102 |
E6_Hrev | 5'-GCGCAAGCTT TTA CAGCTGGGTTTCTCTACGTG -3' | Early protein E6 | 537–559 |
E4_Bfor | 5'-GCGCGGATCC ATG TATTATGTCCTACATCTGTGTTT -3' | Early protein E4 | 3332–3357 |
E4_Hrev | 5'-GCGCAAGCTT CTA TGGGTGTAGTGTTACTATTA-3' | Early protein E4 | 3597–3619 |