Fig. 2

Detection of HPV cervical and nasopharyngeal biopsies using MYO9/GP5 + /6 +  (MY/GP). Representative ethidium bromide-stained 2.5% agarose gel electropherogram of PCR for the identification of HPV L1 region. M = 100 bp molecular ladder; HPV positive samples = 1–12; P1 = positive control for first round PCR; P2 = positive control for nested PCR. The MY primer set amplified a 450 bp fragment within the HPV L1 region, the GP primers amplified a 140 bp fragment within the MY amplified region and the pairing of MY and GP primers further amplified a 190 bp fragment from the 450 bp region. N = negative control