Fig. 4

IGFBP2 strengthens HCC tumorigenic ability in vivo. A The figure of subcutaneous tumor tissue of nude mice. B The weight of subcutaneous tumor tissue of nude mice. **p < 0.01 versus HepG2/pcDNA3.1-Ctr. C The changes of tumor volume of nude mice. **p < 0.01 versus HepG2/pcDNA3.1-Ctr. D Expression of IGFBP2 and EMT related proteins were detected by immunohistochemistry. Scale bars, 100 μm. E Expression of IGFBP2 and EMT related proteins were detected by western blot. F Expression and localization of β-catenin in tumor tissues of nude mice was detected by immunofluorescence. Scale bars, 50 μm. G Co-expression of IGFBP2 and LRP6 in tumor tissues of nude mice were detected by immunofluorescence. Scale bars, 50 μm