Fig. 3

IGFBP2 drives EMT to promote invasion and metastasis in HCC cells via LRP6/ β-catenin signaling pathway. A Expression of β-catenin was detected by western blot. B Expression and localization of β-catenin was detected by immunofluorescence. Scale bars, 50 μm. C The transcript level of β-catenin stimulated by recombinant human IGFBP2 in HepG2 cells was detected by double luciferase reporter gene method. **p < 0.01 versus Ctr. D The interaction of LRP6 with IGFBP2 was detected by immunoprecipitation (CO-IP) after transfection of IGFBP2 plasmid or the vector control in HepG2 cells. E Co-expression of IGFBP2 and LRP6 were detected by immunofluorescence. Scale bars, 50 μm. F Expression of β-catenin and EMT related proteins were detected by western blot after CHIR-99021 treatment