Impact of NLRP3 gene polymorphisms (rs10754558 and rs10733113) on HPV infection and cervical cancer in southern Chinese population

Table 1 Primer Sequence and the Reaction Condition for NLRP3 Polymorphisms

SNP	Primer Sequence	PCR Reaction Conditions			Length
		Initial Step	Melt/Anneal/Extend	Elongate Step
rs10754558	Forward:5’-ACCCAGGCTTTCTATTTGCTTT-3’	94℃ for 3 min	35 cycles of 94℃ for 30 s, 57℃ for 30s, and 72℃ for 30 s	72℃ for 5 min	402 bp
	Reverse:5’-ATGAGGTCACCAAGAGGAACATT-3’				402 bp
rs10733113	Forward:5’-AACCTATCTGTGCCTCACTTGT − 3’				248 bp
	Reverse: 5’-ACTACTTCTTGCGGCCTGTC − 3’				248 bp

ISSN: 1750-9378