Table 1 Sequences of the primers and probes used in this study
Primer name | Sequences (5′−3′) | Product size (bp) |
|---|---|---|
4 A Forward (outer) | AACGCCTCAGAAAAATACCC | 340 |
4B Reverse (outer) | TAAGTGCTGAAACTCTAAWGG | |
4 C Forward (inner) | CAAAAGCATGTGGAGTGAGG | 106 |
4D Reverse (inner) | CACYTTATAATGGTGCTCTGG | |
B19QF | CCATATGACCCAGAGCACCA | 83 |
B19QR | ACCTTTGCCTCCTTTCCACT | |
B19Q Probe | FAM-CCCGCAGCAAGTAGCTGCCA-BHQ | |
ß-Globin F | TGTGTTCACTAGCAACCTCAA | 111 |
ß-Globin R | CTCACCACCAACTTCATCCA | |
ß-Globin Probe | FAM-CCTGAGGAGAAGTCTGCCGTTACTGCC-BHQ |