Evaluating the presence of Mycoplasma hyorhinis, Fusobacterium nucleatum, and Helicobacter pylori in biopsies of patients with gastric cancer

Table 1 Primers used in the PCR assay for detecting β-globin, Mollicutes, Helicobacter pylori, Fusobacterium nucleatum, and Mycoplasma hyorhinis

Primer	Sequence (5′–3′)	Location	Amplicons	Ref
PC03	ACACAACTGTGTTCACTAGC	nt 1575-1594	110 bp	[29]
PC04	CAACTTCATCCACGTTTCAC	nt 1684-1669
GPO3	GGGAGCAAACACGATAGATACCCT	nt 221-245	270 bp	[32]
MGSO	TGCACCATCTGTCACTCTGTTAACCTC	nt 491-465
JW21	GCGACCTGCTGGAACATTAC	nt 691-710	138 bp	[33]
JW22	CGTTAGCTGCATTACTGGAGA	nt 829-809
FNF	CAACACCTAGTAATCATC	nt 2.443.126-2.443.126	653 bp
FNR	CGAATGCTAATACCTATA	nt 2.443.761-2.443.778		[35]
FN-Probe	Cy5-GGCTTCCCCATCGGCATTCC-BHQ	nt 2.443.229-2.443.248	604 bp	[34]
MHRHF	GAACGGGA TGTAGCAATACATTC	nt 18520-18542
MHRHR	AGCGGACTGAAGTTGAGCTTCAG	nt 19124-19102
Mhr-p37-RT-F	TATCTCATTGACCTTGACTAAC	nt 768.070-768.092	89 bp	[15]
Mhr-p37-RT-R	ATTTTCGCCAATAGCATTTG	nt 816.070-835.070
Mhr-p37-RT-Probe	6FAM-CATCCTCTTGCTTGACTACTCCTG-MGBNFQ	nt 774.070-796.070

Target sequences for β-globin amplification – PC03/PC04 (110 bp). Target sequences for 16S rRNA of Mollicutes—GPO3/MGSO (270 bp), H. pylori—JW21/JW22 (138 bp), F. nucleatum—FNF/FNR (653 bp), M. hyorhinis—MHRHF/MHRHR (604 bp), and for p37 gene rRNA of M. hyorhinis—Mhr-p37-RT-F/ Mhr-p37-RT-R / Mhr-p37-RT-Probe (89 bp)

ISSN: 1750-9378