Fig. 4

HPV16 E6 affected the radiosensitivity in HPV + HNSCC via regulating miR-27a-3p/SMG1 axis. a The relative mRNA expression of SMG1 in E6-overexpressing HNSCC cells treated with or without miR-27a-3p inhibitor. b The relative protein expression of SMG1 in E6-overexpressing HNSCC cells treated with or without miR-27a-3p inhibitor. c The colonization of TUNEL (+) HNSCC cells under radiation dose of 4 Gy (left panel). The apoptosis rate was evaluated in E6/SMG1 overexpressing HNSCC cell lines treated with or without miR-27a-3p inhibitor (right panel). d Colony formation assay of E6/SMG1 overexpressing HNSCC cells treated with or without miR-27a-3p inhibitor (left panel). The number of colonies was counted and compared among different groups (right panel). Data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001