Prevalence of human papillomavirus genotypes among women with cervical cancer in Ghana

Table 1 Sequences of synthetic oligonucleotide primers used for HPV genotyping

Primer cocktail	HPV genotype primer	Size of amplicon (bp)	Sequence 5′to 3′)
Consensus primers	GP-E6-3 F		GGGWG KKACT GAAAT CGGT
	GP-E7-5B		CTGAG CTGTC ARNTA ATTGC TCA
	GP-E7-6B		TCCTC TGAGT YGYCT AATTG CTC
Cocktail 1	HPV16f	457	CACAG TTATG CACAG AGCTGC
	HPV16r	457	CATAT ATTCA TGCAA TGTAG GTGTA
	HPV18f	323	CACTT CACTG CAAGA CATAG A
	HPV18r	323	GTTGT GAAAT CGTC GTTTT TCA
	HPV31f	263	GAAAT TGCAT GAACT AAGCT CG
	HPV31r	263	CACAT ATACC TTTGT TTGTC AA
	HPV59f	215	CAAAG GGGAA CTGCA AGAAA G
	HPV59r	215	TATAA CAGCG TATCA GCACC
	HPV45f	151	GTGGA AAAGT GCATT ACAGG
	HPV45r	151	ACCTC TGTGC GTTCC AATGT
Cocktail 2	HPV33f	398	ACTAT ACACAACATT GAACT A
	HPV33r	398	GTTTT TACAC GTCAC AGTGC A
	HPV6_11f	334	TGCAA GAATG CACTG ACCAC
	HPV6_11r	334	TGCAT GTTGT CCAGC AGTGT
	HPV58f	274	GTAAA GTGTG CTTAC GATTG C
	HPV58r	274	GTTGT TACAG GTTAC ACTTG T
	HPV52f	229	TAAGG CTGCA GTGTG TGCAG
	HPV52r	229	CTAA TAGTT ATTTCA CTTAA TGGT
	HPV56f	181	GTGTG CAGAG TATGT TTATT G
	HPV56r	181	TTTCT GTCAC AATGC AATTG C
Cocktail 3	HPV35f	358	CAACG AGGTA GAAGA AAGCA TC
	HPV35r	358	CCGAC CTGTC CACCG TCCAC CG
	HPV42f	277	CCCAA AGTAG TGGTC CCAGT TA
	HPV42r	277	GATCT TTCGT AGTGT CGCAG TG
	HPV43f	219	GCATA ATGTC TGCAC GTAGC TG
	HPV43r	219	CATGAAACTG TAGAC AGGCC AAG
	HPV44f	163	TAAAC AGTTA TATGT AGTGT ACCG
	HPV44r	163	TATCA GCACG TCCAG AATTG AC
Cocktail 4	HPV68f	333	GCAGAAGGCA ACTAC AACGG
	HPV68r	333	GTTTA CTGGT CCAGC AGTGG
	HPV39f	280	GACGACCACT ACAGC AAACC
	HPV39r	280	TTATG AAATC TTCGT TTGCT
	HPV51f	223	GAGTA TAGAC GTTAT AGCAG G
	HPV51r	223	TTTCG TTACG TTGTC GTGTA CG
	HPV66f	172	TTCAG TGTAT GGGGC AACAT
	HPV66r	172	AAACA TGACC CGGTC CATGC

F, f forward, r reverse, B back
Single letter code: W = A/T; K = G/T; R = A/G; Y = C/T; N = A/C/G/T; X = unknown. (Adapted from Soltar et al., [14])

ISSN: 1750-9378