Table 1 List of primers and probes used for the respective genes
Probe | Reverse primer | Forward primer | Genes |
|---|---|---|---|
5'FAM-CAGCACTTCTTTTGAGCACACGGTCG-3'TAMRA | CAGTGGTTTAGGAGGGTTGCTT | TTCCAGAAAATAAATCAGATGGTATGTAA | Rb1 |
5'FAM- TTGCAAAGCATTGGAATCAGACAGCACT- 3'TAMRA | CTCGGCCCTCTTTTCTCTG | GCACTGGCTGATGAGTGTGT | hTERT |
AGCACCGCCGTCTGGTTGGC | CGGCAGTGGCGGTGGTGGTG | FGFR3 | |
5'FAM-CTGTGACTTGCACGTACTCCCCTGCC- -3'TAMRA | AAGACCTGCCCTGTGCAGC | CGTCTGGGCTTCTTGCATTC | TP53 |
5'FAM-CGGCAGACCAGCATGACAGATTTCTACCA-3'TAMRA | GAGGAAGCCTAATCCGCC | CTGGAGACTCTCAGGGTCGAAA | CDKN1A (P21) |
5'FAM-CAAGCTTCCCGTTCTCAGCC- 3'TAMRA | GAAGATGGTGATGGGATTTC | GAAGGTGAAGGTCGTAGTC | GAPDH |