The role of human papillomavirus in head and neck cancer in Senegal
© Ndiaye et al.; licensee BioMed Central Ltd. 2013
Received: 15 October 2012
Accepted: 12 April 2013
Published: 17 April 2013
Exploring the presence and role of human papillomavirus (HPV) in head and neck cancer (HNC) is a necessary step to evaluate the potential impact of HPV prophylactic vaccines.
To assess the prevalence and oncogenic role of HPV in HNC in Senegal.
This is a multicenter cross-sectional study. Paraffin-embedded blocks of cases diagnosed with invasive HNC between 2002 and 2010 were collected from 4 pathology laboratories in Senegal. Presence of HPV DNA was determined by PCR and DEIA, and genotyping performed with LiPA25. Tubulin analysis was performed to assess DNA quality. HPV DNA-positive cases were tested for p16INK4a expression.
A total of 117 cases were included in the analysis: 71% were men, mean age was 52 years old (SD ±18.3), and 96% of cases were squamous cell carcinoma. Analysis was performed on 41 oral cavity tumors, 64 laryngeal tumors, 5 oropharyngeal tumors and 7 pharyngeal tumors. Only four cases (3.4%; 95% CI = 0.9%-8.5%) harbored HPV DNA. HPV types detected were HPV16, HPV35 and HPV45. However, among HPV-positive cases, none showed p16INK4a overexpression.
Our findings indicate that HPV DNA prevalence in HNC in Senegal is very low, suggesting that HPV is not a strong risk factor for these cancers. Additional larger studies are needed to confirm these findings and explore other potential risk factors specific to the region.
KeywordsHead and neck cancer Human papillomavirus Senegal Sub-Saharan Africa
Head and neck cancer (HNC) is the eighth most common cancer worldwide with approximately 650,000 new cases and 350,000 deaths reported each year . In addition to alcohol and tobacco consumption, the latest evaluation on the carcinogenicity of infections and cancer by the International Agency for Research on Cancer established human papillomavirus (HPV) as a carcinogen of the oral cavity and oropharynx .
In sub-Saharan Africa, knowledge on the role of HPV in HNC is very limited. Actually, 84% of the existing information on HPV and HNC is derived from studies in Europe and North America . The aim of our research is to provide original data on the role of HPV in invasive HNC in Senegal.
Material and methods
This is a cross-sectional study designed and coordinated by the Catalan Institute of Oncology in Barcelona and is part of a large international study on HPV in HNC. Four major centers in Dakar, Senegal, have provided consecutive cases diagnosed with HNC from 2002 to 2010: Centre Hospitalier Universitaire A. Le Dantec, Hôpital Principal de Dakar, Hôpital Général de Grand Yoff and the pathology laboratory at Cheikh Anta Diop University. For each specimen, after pathological confirmation of invasive diagnosis, a paraffin tissue section was treated with 250 μl of freshly prepared Proteinase K solution to extract DNA. SPF-10 polymerase chain reaction (PCR) was performed using 10 μl of a 1:10 dilution of the crude DNA extract in a final reaction volume of 50 μl. The amplified PCR products were tested using a probe hybridization step with a cocktail of conservative probes that can recognize around 54 mucosal HPV genotypes using a microtiter plate format for the detection of HPV DNA through a DNA enzyme immunoassay (DEIA) (produced by DDL, Voorburg, the Netherlands). Optical densities (OD450) were read on a microtiter plate reader and categorized as HPV DNA negative, positive, or borderline. After PCR, 10 μl of the DEIA HPV DNA positive amplimers were used to perform the reverse hybridization line probe assay (LiPA25) (version 1: produced by DDL, Voorburg, the Netherlands). The LiPA25 detects 25 high-risk and low-risk HPV types (6, 11, 16, 18, 31, 33, 34, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 66, 68, 70, and 74). Additionally, amplification of the human tubulin gene was performed to determine DNA quality of DEIA negative samples. In HPV DNA-positive samples, p16INK4a overexpression was evaluated using CINtec histology kit (clone E6H4, mtm Laboratories, Heidelberg, Germany) in order to verify the potential causal role of HPV in positive cases. Overall HPV detection percentages and 95% confidence intervals (95% CI) were estimated. Data analyses were performed with STATA 11.0. Ethical approval was obtained from the Ministry of Health of Senegal.
HPV DNA detection in head and neck cancer cases in Senegal by subjects’ characteristics
Cases tested for HPV
HPV positive cases
% (95% CI)
Age at diagnosis (in years)
Period of diagnosis
2002 - 2008
2009 - 2010
Squamous cell carcinoma
HPV DNA, HPV types and p16 INK4a detection in HPV positive cases by head and neck cancer sites and subsites
HNC sites and subsites
Cases tested for HPV
HPV DNA positive
% (95% CI)
HPV35 (N = 1)
Floor of the mouth
Oral cavity unspecified
HPV45 (N = 2)
HPV16 (N = 1)
Hypopharynx or larynx
To our knowledge, this is the first study to explore the presence of viral DNA and cellular protein expression in head and neck tumors in West Africa. HPV DNA was detected in 3.4% (95% CI = 0.9%-8.5%) of studied cases but further analyses testing for p16INK4a expression suggested that HPV was not involved in the oncogenic process of the tumors. Expression of p16INK4a is strongly seen in HPV-associated tumors but nearly absent in HPV-negative carcinomas  and increases in cases where HPV is oncogenically involved due to the interaction of the viral oncoprotein E7 with pRb .
Similar low HPV prevalences have been reported in the African-American community in the USA. In a prospective study conducted by Settle et al. , overall HPV positivity was 4% in black patients diagnosed with oropharyngeal cancer versus 34% in white patients. Weinberger et al.  found 0% HPV-active HNC in black patients compared to 21% in white patients after immunohistochemical testing with p16INK4a. Both studies agree on significant ethnic and biological disparities in HPV prevalence, after adjusting for clinical and sociodemographic characteristics, and support the validity of our study. Further studies are needed to elucidate the mechanism of HPV clearance in African-American and African populations, which share common ancestral origins.
These findings are very different from what has been reported previously in other regions of the world. In a systematic review by Kreimer et al.  compiling data on 60 studies focusing on HNSCC, HPV DNA was detected overall in 26% of cases. Reported site-specific HPV prevalence was 23.5% (95% CI = 21.9-25.1%) in oral SCC, 35.6% (95% CI = 32.6-38.7%) in oropharyngeal SCC and 24.0% (95% CI = 21.8-26.3%) in laryngeal SCC . The highest detection rates were found in Asia, followed by the USA and Nordic countries .
Low HPV prevalence in invasive HNC in Senegal suggest that other established risk factors such as alcohol and tobacco consumption may play a more significant etiological role than HPV infection in HNC. However, the prevalence of tobacco use in Senegal is relatively low with estimates of 19.9% in men and 1.3% in women . Alcohol consumption is also negligible, especially in women, since Senegal is a predominantly Muslim Country with 95% of the population practicing this religion. Thus, the magnitude of the implication of these two classical risk factors in the development of HNC is probably small given the social norms. Therefore, we hypothesize that environmental factors and eating habits may contribute more importantly to the development of HNC.
For example, indoor air pollution causes 6,300 deaths each year in Senegal due to daily exposure to smoke from open burning of wood and charcoal in homes . More than 80% of the households use either wood or charcoal as cooking fuels in peri-urban and rural Senegal. Moreover, burning incense to deodorize and heat the indoor is a widely practiced cultural habit. The aforementioned are potential risk factors for laryngeal and nasopharyngeal cancers as they expose individuals to smoke and dangerous emissions of particles such as CO2 and CO . According to the WHO, 3.7% of the burden of disease in developing countries can be attributed to indoor air pollution .
Another potential risk factor could be the consumption of “ataya”, a strong and bitter hot tea comparable to “yerba mate” in South America, which has been associated with an increased risk of developing cancer of the oral cavity, larynx and esophagus . On average, at least three rounds of “ataya” are served twice a day in Senegal. The fact that this tea is drank at very hot temperature and consumed with slurps can contribute to an increased risk of cancer.
Poor oral hygiene is another potential risk factor that has been documented  and could play a non-negligible role in Senegal. A study in 330 Senegalese university students has shown poor dental health and the need to improve prevention programs . This study was conducted in an educated cohort, which suggests that dental issues may be worse in uneducated, low socio-economic populations with limited access to dental care. Moreover, chewing of kola nuts (Cola acuminate) has also been reported to increase carcinogenesis potential of tobacco in smokers in Nigeria by promoting palatal mucosa keratinization . Consumption of kola nuts is also widespread in Senegal.
Occupational exposure such as jobs in the construction, metal, textile, ceramic, logging, and food industries has been associated with the development of laryngeal cancer . In Senegal, protective masks and safety rules are not applied, as workplace safety regulations are not reinforced mainly due to the fact that the informal sector provides most of the jobs. This reality puts workers in a difficult predicament whereby they expose themselves to hazardous materials.
Our study has some methodological limitations. At sample collection stage, some cases were identified eligible for the study but corresponding blocks were not found as they were sent to laboratories abroad for histopathological evaluation. Additionally, several archival records were lost in one of the main laboratories due to a fire. However, these issues affected all cases regardless of patient characteristics or diagnosis. Another major limitation is the lack of individual data on risk factors: no information on patients besides their gender, age and pathological information were available in the registries. Finally, the small sample size (n = 5) for oropharyngeal tumors may have affected our positivity rate. It is recognized that the highest associations of HPV in HNC have been shown in the oropharynx, particularly in the tonsils, with a positivity of 57% to 82% compared to 0.8% to 9% in other anatomic sites .
Our study has several strengths comprising the combined use of highly sensitive assays for HPV DNA detection and a marker of HPV related transforming process such as p16INK4a expression. Additionally, we evaluated the quality of our specimens by means of cellular tubulin detection and subsequently excluded the HPV DNA negative and tubulin negative cases from the statistical analyses. Lastly, all of the study cases were selected from the main anatomy and pathology laboratories in Senegal. Thus, our cohort is representative of the target population as the vast majority of patients are diagnosed in participant centers.
In Senegal, HPV prevalence in HNC is very infrequent and HPV infection is not associated with carcinogenesis. Therefore, HPV prophylactic vaccines would not have any impact on HNC incidence. Our findings need to be further validated with supplementary studies that include larger case series and the assessment of region-specific risk factors. Additional data will allow to close the gap of knowledge between Western countries and the sub-Saharan African region and to assist health authorities in implementing public health strategies.
We are grateful to Dr. Magib Gaye and Dr. Ibrahima Thiam for their contribution to data collection.
The study was partially supported by: Spanish public grants from the Instituto de Salud Carlos III (grants FIS PI081535, PI11 02104, RCESP C03/09, RTICESP C03/10, RTIC RD06/0020/0095 and CIBERESP), the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR 2005SGR 00695), and Sanofi Pasteur MSD & Merck & Co, Inc., who had no role in the data collection, analysis or interpretation of the results. CN received a training scholarship from Fonds de Recherche en Santé du Québec and was also supported by an HPV infection and associated diseases team grant from the Canadian Institutes of Health Research (CIHR) held by HT.
Role of funding sources
The sponsors did not have any role in the study design, collection, analysis or interpretation of the data.
- Ferlay J, Shin H-R, Bray F, Forman D, Mathers C, Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 2010, 127 (12): 2893-2917. 10.1002/ijc.25516.PubMedView Article
- IARC: Monographs on the evaluation of carcinogenic risks to humans, vol. 100. A review of human carcinogens. Part B: Biological agents. 2010, Lyon, France: International Agency for Research on Cancer
- Kreimer AR, Clifford GM, Boyle P, Franceschi S: Human papillomavirus types in head and neck squamous cell carcinomas worldwide: a systematic review. Cancer Epidemiol BiomarkersPrev. 2005, 14 (2): 467-475. 10.1158/1055-9965.EPI-04-0551.View Article
- Jung AC, Briolat J, Millon R, de Reyniès A, Rickman D, Thomas E: Biological and clinical relevance of transcriptionally active human papillomavirus (HPV) infection in oropharynx squamous cell carcinoma. Int J Cancer. 2010, 126 (8): 1882-1894.PubMed
- Smeets SJ, Hesselink AT, Speel E-JM, Haesevoets A, Snijders PJF, Pawlita M: A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen. Int J Cancer. 2007, 121 (11): 2465-2472. 10.1002/ijc.22980.PubMedView Article
- Settle K, Posner MR, Schumaker LM, Tan M, Suntharalingam M, Goloubeva O: Racial Survival Disparity in Head and Neck Cancer Results from Low Prevalence of Human Papillomavirus Infection in Black Oropharyngeal Cancer Patients. Cancer Prev Res. 2009, 2 (9): 776-781. 10.1158/1940-6207.CAPR-09-0149.View Article
- Weinberger PM, Merkley MA, Khichi SS, Lee JR, Psyrri A, Jackson LL: Human Papillomavirus-Active Head and Neck Cancer and Ethnic Health Disparities. Laryngoscope. 2010, 20: 1531-View Article
- Institut Català d'Oncologia: Human Papillomavirus and Related Cancers in Senegal. Summary Report Update. 2010, Available at http://www.who.int/hpvcentre
- World Health Organization: Indoor Air Pollution, Health and the Burden of Disease. 2010,http://www.who.int/indoorair/en/,
- Dasanayake AP, Silverman AJ, Warnakulasuriya S: Maté drinking and oral and oro-pharyngeal cancer: a systematic review and meta-analysis. Oral Oncol. 2010, 46 (2): 82-86. 10.1016/j.oraloncology.2009.07.006.PubMedView Article
- Guha N, Boffetta P, WünschFilho V, ElufNeto J, Shangina O, Zaridze D: Oral health and risk of squamous cell carcinoma of the head and neck and esophagus: results of two multicentric case–control studies. Am J Epidemiol. 2007, 166 (10): 1159-1173. 10.1093/aje/kwm193.PubMedView Article
- Faye D, Cisse D, Mbodj EB, Lo CMM: Epidemiologic study of dental caries among students on the campus of the University of Dakar. Odontostomatol Trop. 2007, 30 (119): 29-36.PubMed
- da Lilly-Tariah OB, Somefun AO, Adeyemo WL: Current evidence on the burden of head and neck cancers in Nigeria. Head Neck Oncol. 2009, 1: 14-10.1186/1758-3284-1-14.PubMedPubMed CentralView Article
- Paget-Bailly S, Cyr D, Luce D: Occupational exposures and cancer of the larynx-systematic review and meta-analysis. J Occup Environ Med. 2012, 54 (1): 71-84. 10.1097/JOM.0b013e31823c1343.PubMedView Article
- Snow AN, Laudadio J: Human papillomavirus detection in head and neck squamous cell carcinomas. Adv Anat Pathol. 2010, 17: 394-403. 10.1097/PAP.0b013e3181f895c1.PubMedView Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.