Figure 3

Demonstration of the specificity of 1G3 binding to galectin-9 by a capture ELISA assay. 1G3 and irrelevant mouse IgG were adsorbed on an ELISA plate and then incubated with various types of biotynilated human galectins (hGAL 1,2,3,4,7,8 and hG9 and hG10) or the M-isoform of murine galectin-mG9. Both human and murine galectin-9 are captured by 1G3 in contrast with other galectins which do not exhibit significant binding to 1G3.