Single-tube multiplex PCR using type-specific E6/E7 primers and capillary electrophoresis genotypes 21 human papillomaviruses in neoplasia

Dictor, Michael; Warenholt, Janina

doi:10.1186/1750-9378-6-1

Table 1 HPV primer and product characteristics

From: Single-tube multiplex PCR using type-specific E6/E7 primers and capillary electrophoresis genotypes 21 human papillomaviruses in neoplasia

HPV type	Genbank accession no.		E6/E7 Primers¹		Product's molecular size (nt)	Product's electrophoretic size (nt)²
		Forward	5' label	Reverse
39	M62849	ACA GTG TCG ACG GTG CTG GA	FAM	GCT TTG GTC CAC GCA TAT CTG A	94	92
70	U21941	ACA GTG CCG ACA CTG CTG GA	NED	GGC CGT GGT CCA TGC ATA TT	95	94
68	Y14519	ACA GTG TCG S CA CTG CTG GA	HEX	GGG CTT TGG TCC ATG CAT AGT	95	95
18	X05015	AGT GCC ATT CGT GCT GCA AC	FAM	ATG TTG CCT TAG GTC CAT GCA T	98	96
56	X74483	TGG TTG GAC CGG GTC ATG T	HEX	CGT CTT GCA GCG TTG GTA CTT T	103	100
45	X74479	GGA CAG TAC CGA GGG CAG TGT A	NED	CCG GGG TCC ATG CAT ACT TAT	107	106
33	M12732	AAT ATT TCG GGT CGT TGG GC	FAM	AAC GTT GGC TTG TGT CCT CTC A	109	107
35	M74117	GGT GGA CAG GTC GGT GTA TGT C	HEX	GTT GCC TCG GGT TCC AAA TC	120	116
66	U31794	ACC GGG TCA TGT TTG CAG TGT	NED	CGT TTG CGG TGC AAG TTC TAA T	122	120
6/6b	AF092932 X00203	CCA AGG CR C GGT TCA TAA AGC	FAM	GGG TCT GGA GGT TGC AGG TCT A	123	120
6a	L41216	CCA AGG CGC GGT TTA TAA AGC	FAM	GGG TCT GGA GGT TGC AGG TCT A	123	120
31	J04353	GTG GAC AGG ACG TTG CAT AGY A	HEX	GGT CAG TTG CCT CAG GTT GCA	124	121/203³
26	X74472	GGG CAG TGG AAA GGG TTG TGT	NED	GGT TGC GGC ACC AGA TCT AGT A	128	126
51	M62877	AAT GCG CTA ATT GCT GGC AA	HEX	TGC TCG TAG CAT TGC AAG TCA A	143	142
53	X74482	ATA TGT GGA CCG GGT CGT GC	FAM	GGC ATT GCA GGT CAA TCT CAG T	143	143
82	AB027021	ACG GGA CAG TGT GCA AAT TGC	HEX	TGC TCG TAG CAT TGC AAG TCA A	150	150
52	X74481	GTT GGA CAG GGC GCT GTT C	NED	CCT CCT CAT CTG AGC TGT CAC C	166	167
59	X77858	ACA GTG TCG TGG GTG TCG GA	FAM	TGC TCG TAG CAC ACA AGG TCA A	169	169
58	D90400	AGG GCG CTG TGC AGT GTG T	HEX	CAT CCT CGT CTG AGC TGT CAC A	172	172
11	M14119	GGG AAA GGC ACG CTT CAT AAA	NED	TGT CCA CCT TGT CCA CCT CAT C	190	190
16	K02718	GTG GAC CGG TCG ATG TAT GTC T	HEX	TCC GGT TCT GCT TGT CCA GC	209	210
73	X94165	AAC AGT GGA CCG GAC GCT GT	FAM	GGC AAG GCA TAC TGT GCA CTG A	258	260
β-globin	NG000007	GAA GAG CCA AGG ACA GG TAC	HEX	CAA CTT CAT CCA CGT TCA CC	268	268

¹ Each ambiguous base code S, R and Y yields two different primer sequences. HPV types 51 and 82 share identical reverse primers.
² Default colors: FAM, blue; NED, black; HEX, green.
³ Secondary HEX peak at 203 nt is due to cross-annealing to the HPV16 reverse primer.

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ISSN: 1750-9378

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