Volume 5 Supplement 1

Proceedings of the 12-th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI)

Open Access

Persistence of anal squamous intraepithelial lesions and anal HPV infection in HIV-infected patientsdespite immune restoration under cART

  • C Piketty1Email author,
  • E Lanoy2,
  • A Si-Mohamed1,
  • B Cochand-Priolet3,
  • S Trabelsi2,
  • P-M Girard4,
  • R Tubiana5,
  • L Abramowitz6,
  • E Tartour1, 8,
  • C Rouzioux7, 8,
  • L Weiss1, 8,
  • D Costagliola2, 5 and
  • The Valparaiso Study Group
Infectious Agents and Cancer20105(Suppl 1):A59

DOI: 10.1186/1750-9378-5-S1-A59

Published: 11 October 2010

Background

A high prevalence of anal squamous intraepithelial lesions (ASIL) and HPV infection have been observed in HIV-infected MSM in the pre-cART era. To date, the impact of cART on the natural history of HPV infection and ASIL is poorly documented.

Methods

94 HIV-infected MSM naïve of cART were enrolled in a longitudinal study before starting a first-line regimen of cART. Each patient provided anal samples for cytology, histology, and HPV DNA testing at baseline, month 12, and month 24 of cART. HPV DNA was detected by real-time PCR and Roche Linear Array assay. Anal cytologic was processed by the Thin Prep™ method (Hologic). CD4+ and CD8+ T cell responses to HPV-16 E6 and E7 proteins were measured in a subgroup of individuals exhibiting HPV-16 anal infection at inclusion.

Results

Prevalence of low-grade SIL, high-grade SIL, and HPV infection was similar at M12 compared to baseline. Among patients with normal cytology and/or histology at baseline, 44% progressed to SIL at M12 whereas 31% of patients with ASIL at baseline exhibited a regression at M12. Specific anti-HPV CD4 T cell responses were mostly undetectable both at baseline and M12. (Table 1 and 2)
Table 1

The median age of the patients was 39.7 years (33.2-43.5). Baseline and month 12 cytologic and/or histologic results.

 

CD4/mm3 median (Q1-Q3)

Plasma HIV RNA log10 copies/mL

VL <50

Prior AIDS event

Visible lesion

Presence of condyloma

Anal SIL

Low-grade SIL; High-grade SIL

Baseline

299 (242 – 342)

4.8 (4.17 – 5.26)

1%

4 (4%)

40/94 (43%)

23/94 (25%)

51 (54%)

30 (32%); 8 (9%)

M12

500 (411 – 575)

1.6 (1.6 – 1.6)

93%

 

25/71 (35%)

5/71 (7%)

41 (58%)

24 (34%); 10 (14%)

Table 2

Baseline and month 12 virological results.

 

Number of HPV

Number of high-risk and low-risk type

High risk HPV

HPV-16

HPV-18

HPV-16 DNAlog10 copies/106 cells

Baseline

5 (2 – 7)

3 (2 – 5); 2 (1 – 4)

83 (90%)

49 (53%)

28 (30%)

6.1 (5.3 – 7.1)

M12

5 (2 – 6)

3 (1 – 4); 2 (1 – 4)

59 (87%)

28 (41%)

15 (22%)

6.1 (2.0 – 7.2)

At month 12, prevalence of anal HPV DNA detection was similar than at baseline. High-risk HPV was detected at month 12 in 92% of the patients with high-risk HPV infection at baseline. Low-risk HPV was detected at month 12 in 91% of the patients with low-risk HPV infection at baseline. HPV-16 and HPV-18 were detected at month 12 in 13% and 3.7% of patients with no HPV-16 and HPV 18 infection at baseline, respectively. HPV-16 was detected in 100% and 70% of high-grade SIL at baseline and month 12, respectively.

Conclusion

Our results demonstrate a high prevalence and incidence of ASIL and anal HPV infection in HIV-infected MSM despite CD4 recon-stitution under cART. These data suggest that all HIV-positive MSM, even under antiretroviral therapy, remain at risk of anal SIL.

Declarations

Acknowledgements

This article has been published as part of Infectious Agents and Cancer Volume 5 Supplement 1, 2010: Proceedings of the 12th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI).The full contents of the supplement are available online at http://www.biomedcentral.com/1750-9378/5?issue=S1.

Authors’ Affiliations

(1)
(2)
INSERM U943 - UPMC UMR S943
(3)
(4)
(5)
(6)
(7)
(8)
Université Paris 5

Copyright

© Piketty et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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