Analysis of colorectal cancers for human cytomegalovirus presence

Table 2 Primers used for HCMV DNA amplification by nested PCR

primer	sequence	amplicon length	*UL55* gene position
E-1	TCCAACACCCACAGTACCCGT	267 bp	655 to 675
E-2	CGGAAACGATGGTGTAGTTCG		902 to 922
I-1	GTCAAGGATCAGTGGCACAGC	182 bp	685 to 705
I-2	GTAGCTGGCATTGCGATTGGT		847 to 867

Primers used for amplification of the HCMV glycoprotein B (UL55) gene in nested PCR. External primers amplify a 267 bp fragment, while internal primers amplify a 182 bp fragment

ISSN: 1750-9378