H. pylori-infection and antibody immune response in a rural Tanzanian population
© Mbulaiteye et al; licensee BioMed Central Ltd. 2006
Received: 18 July 2006
Accepted: 14 September 2006
Published: 14 September 2006
Helicobacter pylori (H. pylori) infection is ubiquitous in sub-Saharan Africa, but paradoxically gastric cancer is rare.
Sera collected during a household-based survey in rural Tanzania in 1985 were tested for anti-H. pylori IgG and IgG subclass antibodies by enzyme immunoassay. Odds ratios (OR) and confidence intervals (CI) of association of seropositivity with demographic variables were computed by logistic regression models.
Of 788 participants, 513 were aged ≤17 years. H. pylori seropositivity increased from 76% at 0–4 years to 99% by ≥18 years of age. Seropositivity was associated with age (OR 11.5, 95% CI 4.2–31.4 for 10–17 vs. 0–4 years), higher birth-order (11.1; 3.6–34.1 for ≥3rd vs. 1st born), and having a seropositive next-older sibling (2.7; 0.9–8.3). Median values of IgG subclass were 7.2 for IgG1 and 2.0 for IgG2. The median IgG1/IgG2 ratio was 3.1 (IQR: 1.7–5.6), consistent with a Th2-dominant immune profile. Th2-dominant response was more frequent in children than adults (OR 2.4, 95% CI 1.3–4.4).
H. pylori seropositivity was highly prevalent in Tanzania and the immunological response was Th2-dominant. Th2-dominant immune response, possibly caused by concurrent bacterial or parasitic infections, could explain, in part, the lower risk of H. pylori-associated gastric cancer in Africa.
In sub-Saharan Africa, Helicobacter pylori (H. pylori) infection is ubiquitous, with seroprevalence reaching 90% or higher in many populations . H. pylori is transmitted from person-to-person, and transmission risk is high in populations of low socioeconomic status, poor hygiene, and limited access to clean water [2–4]. The most severe consequence of chronic H. pylori infection is gastric adenocarcinoma . However, gastric cancer rates vary widely worldwide and correlate imperfectly with H. pylori seroprevalence . For example, seroprevalence reaches 80% by 5 years of age in sub-Saharan Africa [6, 7], highlighting the particularly young age of infection acquisition, and therefore duration of infection. However, age-standardized gastric cancer incidence rates are relatively low at 2–21 per 100,000 person-years for both males and females .
In Japan, seroprevalence increases more gradually with age to a prevalence of 40% to 70% among adults [9, 10], but the age-standardized gastric cancer incidence rates are substantially higher, ranging from 65–92 and 24–39 per 100,000 person-years among males and females, respectively . By comparison, seroprevalence estimates in the U.S. range from 10 to 20% among adults [10, 11], with the age-standardized gastric cancer incidence rates being 6.6 and 2.6 per 100,000 person-years among white males and females, respectively .
These statistics highlight a paradoxical deficit of gastric cancer cases in sub-Saharan Africa, compared to Western countries after controlling for age, the so-called "African enigma" . Gastric cancer deficits may be artifactual, due to incomplete case ascertainment and competing mortality [13, 14]; however, those reasons do not explain why gastric cancer rates vary in African populations that have comparable access to medical care. Specifically, populations residing in mountainous areas tend to have a higher relative frequency of gastric cancer as compared to populations residing in lowland areas .
Variation in gastric cancer rates within Africa, and elsewhere, suggests the presence of modifying factors on H. pylori-associated gastric cancer risk. In other words, outcomes of H. pylori infection could be influenced by bacterial, host, diet, or other environmental factors. One hypothesis, based on animal studies, posits that bacterial and/or parasitic infections modulate H. pylori-induced gastric cancer risk , perhaps by altering the quality of H. pylori-induced mucosal immunity in the stomach [17, 18]. H. pylori infection is thought to cause gastric cancer by eliciting vigorous T-helper (Th1) pro-inflammatory cellular immune responses in gastric mucosa  and the resulting mucosal injury is mediated by pro-inflammatory cytokines and oxygen radicals secreted by infiltrating chronic inflammatory cells [16, 19]. Parasites and, to a lesser extent, certain bacterial infections , elicit Th2 instead of Th1-dominant immune responses to thwart their elimination  and could plausibly modulate H. pylori-induced immune response towards one less damaging to the gastric mucosa.
We hypothesized that persons living in high H. pylori-prevalence areas with low gastric-cancer incidence in Africa would therefore have Th2-type dominant H. pylori-specific responses. To test this hypothesis, we evaluated H. pylori seropositivity and H. pylori-specific IgG subclass antibodies in a rural population in northern Tanzania, where H. pylori infection was expected to be endemic and gastric cancer incidence is thought to be low.
Subject selection and serological methods
The study subjects were residents of the North Mara District, located on the eastern shores of Lake Victoria in Tanzania, who participated in human immunodeficiency virus (HIV) serological surveys from May through June, 1985 . Participants provided individual verbal consent, and parents provided verbal consent for their children, to participate in serological surveys. Institutional Review Boards gave ethical approval for the study. Participants were recruited from households, defined as compounds where individuals shared meals and had one person designated as head. As previously observed , polygamy was frequently practiced in this population and headmen often had several wives. The households were randomly selected from nine villages located either on hills (n = 5) or in valleys (n = 4). Participants provided sociodemographic information and gave a blood sample . The samples were stored at -80°C and were thawed once before current testing. Samples from all but 10 participants enrolled in the original study were available for H. pylori serologic testing.
Anti-H. pylori antibodies were measured using an IgG enzyme immunoassay (EIA) as previously reported [22–24]. This assay has been validated in various populations, including those from Africa and was shown to have high sensitivity (89–96%) and specificity (92–97%), using biopsy-proven H. pylori gastric mucosa infection as the gold standard [22, 23]. The tests were run in triplicate using a standard 96-well microtiter plate and placing the plates on a Benchmark microplate reader (BioRad, Hercules, CA). EIA cut-off values were derived using known H. pylori-positive and negative control sera in which OD values < 0.8 were considered to be negative, OD values >1.3 were considered positive, and OD values between 0.8 and 1.3 were considered to be indeterminate, as previously described [22, 24]. To determine the IgG antibody subclasses (IgG1 or IgG2) in seropositive individuals, as a marker of Th1/2-type cellular response , we used IgG subclass EIAs to mouse anti-human IgG1 and mouse anti-human IgG2 conjugated to HRP (Zymed Laboratory, San Francisco, CA). In brief, pooled serum was used as a reference standard for the IgG subclasses. This standard was titrated using each of the IgG subclass antibodies (i.e. mouse anti human IgG1 and mouse anti human IgG2) to determine the highest dilution at which reactivity could still be detected and also remain linear on a standard curve [22, 23, 26]. The maximal dilution for both IgG1 and IgG2 subclasses was 1:25,600. The serum was assigned an arbitrary ELISA unit of 1 at this dilution. The ELISA was performed as previously stated using positive controls diluted to 1:25,600 based on the previously mentioned standard curve and double the concentration at 1:12,800 dilution. The IgG subclass unit was calculated as ratio of the OD490 of the individual sample to the OD490 of the standard (i.e., IgG subclass unit = sample OD490/reference OD490). These assays were modified to determine the optimal concentration of antigen in serum and IgG conjugate that discriminated between H. pylori-positive and H. pylori-negative samples. Each plate incorporated H. pylori-specific IgG subclass positive and negative control samples. OD values for IgG1 and IgG2 subclasses were normalized using standards previously reported . We calculated the ratio of IgG1/IgG2 subclasses to determine phenotype of Th1/2-type cellular immune response. Using a previously established criteria to determine Th1 vs. Th2-type response in African and western populations [27, 28], an IgG1/IgG2 ratio >1.0 suggests a Th2-(IgG1)-dominant cellular immune response and a ratio ≤ 1.0 suggests a Th1-(IgG2)-dominant response.
Associations between sociodemographic variables (sex, age, village location) and H. pylori seropositivity were determined using Chi-square or Fisher's exact tests. Because of the high H. pylori seroprevalence, we grouped persons with indeterminate status with seronegative individuals for the analysis. Odds ratios (OR) and 95% confidence intervals (CI) of association of H. pylori seropositivity with age, sex, birth-order were estimated using logistic regression models (PROC GENMOD in SAS 9.1 software package; SAS Institute). Because children are more likely to acquire infection from an infected older sibling, we also estimated the association between seropositivity of a younger child with status of the next-older sibling on whom samples were available. We accounted for intra-familial or intra-household correlations among observations by using generalized estimating equations (25). We used independence working correlation matrices in the computations and checked results by also using equi-correlated working correlations that assumed that members in the same family would have equal correlations. Both methods yielded similar results, so only the results using independent working correlations are presented. Values of IgG1/IgG2 ratio among seropositive individuals were log-transformed to obtain a normal distribution. We assessed the relationship between the log-transformed IgG1/IgG2 ratio and age in linear regression models. Age was used in the categories 0–4, 5–9, 10–17, and 18+ years. A two-sided p-value < 0.05 was considered statistically significant.
Frequency and risk of H. pylori seropositivity among persons residing in rural Tanzania (May to June, 1985)
No. H. pylori positive/Total No. (%)
Age group, years*
Next-older sibling ±
Location of village
We observed extremely high H. pylori seroprevalence in a rural population in northern Tanzania. Seropositive individuals showed a high IgG1/IgG2 ratio, suggestive of Th2-dominant H. pylori-specific immune responses. Our cross-sectional study confirms high H. pylori seropositivity in rural Tanzania, and shows that seropositivity increases steeply with age in childhood and is associated, among children, with higher birth-order and with having a seropositive next-older sibling. These findings are consistent with prior studies of H. pylori infection in highly endemic areas [2, 5]. A novel finding from our study is the Th2-dominant H. pylori-specific immune responses, which were strongest in children but were present also in adults. As assessed by IgG subclass antibodies, the Th2-dominant immune responses to H. pylori infection observed in this rural Tanzanian population contrasts to Th1-dominant responses reported in Western populations [27, 29]. We speculate that Th2-dominant H. pylori-specific immune responses in this population can be modulated by concurrent infection with parasitic or bacterial infections as has been observed in animal models [16, 18]. Th2-dominance rapidly increased with age and peaked between 3–7 years. This trend with age may reflect a tendency for younger children to have a Th2-dominant pattern or may be due to a high frequency and burden of parasitic and bacterial infections among children, or both. Parasite egg and/or worm burden for schistosomiasis and soil-transmitted helminths have been shown to peak at young ages in this population .
Our study highlights one aspect of the gastric cancer paradox in Africa, i.e., early acquisition of H. pylori infection in childhood and persistence of seropositivity into adulthood in an area where gastric cancer is relatively rare. Competing mortality (causing deficits in the elderly population ≥ 60 years) has been advanced as one explanation for the lower age-sex specific gastric cancer incidence in sub-Saharan Africa compared to developed countries. However, this explanation is not supported by data from South Africa where life expectancy prior to the AIDS epidemic was 63 years . Investigators at Shirati Hospital, North Mara District, who serve our study population and are actively engaged in cancer research , previously reported gastric cancer to be rare. In an analysis of 279 malignancies seen at the Hospital from 1952–1965, only 12 (4.3%) were diagnosed as gastric cancer . Conversely, gastric cancer is relatively more frequent on the slopes of Mount Kilimanjaro, where it contributes ~15% of malignancies . Moreover, there are similar reports of higher gastric cancer rates in mountainous or dry areas elsewhere in Africa [15, 35, 36]. The variation in relative frequency of gastric cancer in the context of similar H. pylori prevalence, similar access to medical care, and similar life expectancy has led to the hypothesis that environmental co-factors, perhaps diet (nitrates or fresh fruits and vegetables), are important. However, no dietary factors have been convincingly implicated in Africa. An alternative hypothesis posits that enteric or other parasites may influence gastric cancer risk . This hypothesis is based on the observations in mice that Helicobacter-induced gastric atrophy, a precursor lesion to gastric cancer, improved in mice concurrently infected with enteric parasites but not in mice that were not. Improvements were associated with concomitant down-regulation of the pro-inflammatory responses due to a shift from Th1 toward Th2-dominant cellular immune response in mice with concurrent enteric parasite infection . Our finding of Th2-dominant immune response in H. pylori infected persons from northern rural Tanzania, where gastric cancer is relatively rare, is consistent with this hypothesis. Although we lack data on parasite or colonic bacterial infections, it is reasonable to assume that people who live in remote rural African villages without access to running water or other amenities, such as ours, are frequently infected with parasitic and/or bacterial conditions in addition to H. pylori [30, 37].
The role of Th1/2 immunity and H. pylori-induced gastric cancer has been investigated in two prior studies. In one conducted in Soweto, South Africa, where gastric cancer incidence is low, found Th2-dominant H. pylori-specific immune responses in blacks with gastric symptoms  whereas study of symptomatic white subjects from Austria and Germany, where gastric cancer incidence is high, demonstrated Th1-dominant responses . The authors suggested that the Th2-dominant responses in blacks were likely induced by co-infection with parasites, which they postulated may modulate gastric risk cancer among Africans. In the other study conducted in Columbia in persons from low and mountainous regions where gastric cancer incidence is low and high, respectively, evaluated H. pylori seropositivity and parasitic infections. Persons from Tumaco, a low lying area where gastric cancer incidence is low, had Th2-dominant immune responses and a higher prevalence of helminth infections . Conversely, those from Pasto, a high altitude area where gastric cancer incidence is high, had predominantly Th1-dominant immune responses and a lower prevalence of helminth infections . The authors attributed differences in Th1 or Th2-dominant responses across the low vs. mountainous regions to differences the prevalence of helminth infections and suggested that parasitic infection may modify H. Pylori-induced gastric cancer risk in Columbia. Thus, those studies and ours lend support to the hypothesis that Th1/2 immunity modulated by parasite infections may influence the risk of H. pylori-associated gastric cancer in diverse environments [15, 28, 35, 36].
Our study has some limitations. H. pylori infection was measured using serological assays, and some subjects may have been misclassified. The finding of almost universal seropositivity among adults is surprising because we would have expected a small proportion of individuals to be seronegative due to loss of bacterial colonization that occurs with age in chronically infected persons or with development of chronic gastric atrophy. We lacked endoscopy data on the state of gastric mucosa (presence of ulcer, gastric atrophy or not) and therefore are unable to draw conclusions about the relationship between Th2 dominance and severity of gastric mucosal inflammation. Direct measurement of pepsinogen levels could have also provided some information on the presence or not of gastric atrophy, but the volume of residual samples from the study population were inadequate for additional testing. Furthermore, our study was cross-sectional so we cannot infer temporality of associations demonstrated. We note that our "highland" area villages were in low hills, not mountainous areas, thus our study does not provide data for Th1 vs. Th2 responses in low vs. mountainous regions in Africa. The use of IgG subclasses assays as markers for type1/2-dominant immune response in a general population in Africa is novel, and our study provides valuable baseline data for sub-Saharan Africa. Finally, IgE measurements would strengthen our results on the role of parasites, but we lacked a validated assay to perform IgE studies.
To conclude, H. pylori seropositivity was highly prevalent in rural Tanzania. Seropositive persons showed a Th2-dominant immune response to H. pylori infection, which may be due to effects of concurrent infection with parasites and/or bacterial infections. We speculate that the shifts in immune response from Th1 responses to Th2 responses in early childhood and persistence of that immune profile into adulthood may partially explain the paradoxically lower gastric cancer risk in highly H. pylori-endemic Africa.
We thank participants in the survey and Tanzanian authorities for permitting this work to be done. We are grateful to Frances Yellin (Computer Services, Ltd, Rockville) for preparing the data files, to Violet Devairakkam (Research Triangle Institute, Rockville) for handling the samples, to Dexter Thompson (Division of Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Emory University School of Medicine) for performing H. pylori serology, and to James J. Goedert for comments on the manuscript. This project was funded by the Intramural Research Program of the National Cancer Institute, National Institutes of Health, Department of Health and Human Services, under Purchase Order number 263-NQ-320842.
- Schistosomes, liver flukes and Helicobacter pylori. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Lyon, 7-14 June 1994. IARC Monogr Eval Carcinog Risks Hum. 1994, 61: 1-241.Google Scholar
- Goodman KJ, Correa P: Transmission of Helicobacter pylori among siblings. Lancet. 2000, 355: 358-362. 10.1016/S0140-6736(99)05273-3.PubMedView ArticleGoogle Scholar
- Rothenbacher D, Bode G, Berg G, Gommel R, Gonser T, Adler G, Brenner H: Prevalence and determinants of Helicobacter pylori infection in preschool children: a population-based study from Germany. Int J Epidemiol. 1998, 27: 135-141. 10.1093/ije/27.1.135.PubMedView ArticleGoogle Scholar
- Teh BH, Lin JT, Pan WH, Lin SH, Wang LY, Lee TK, Chen CJ: Seroprevalence and associated risk factors of Helicobacter pylori infection in Taiwan. Anticancer Res. 1994, 14: 1389-1392.PubMedGoogle Scholar
- Holcombe C, Umar H, Lucas SB, Kaluba J: Low incidence of clinically significant gastroduodenal pathology despite a high incidence of Helicobacter pylori infection. Trans R Soc Trop Med Hyg. 1994, 88: 569-571. 10.1016/0035-9203(94)90166-X.PubMedView ArticleGoogle Scholar
- Lindkvist P, Enquselassie F, Asrat D, Nilsson I, Muhe L, Giesecke J: Helicobacter pylori infection in Ethiopian children: a cohort study. Scand J Infect Dis. 1999, 31: 475-480. 10.1080/00365549950163996.PubMedView ArticleGoogle Scholar
- Thomas JE, Dale A, Harding M, Coward WA, Cole TJ, Weaver LT: Helicobacter pylori colonization in early life. Pediatr Res. 1999, 45: 218-223.PubMedView ArticleGoogle Scholar
- Parkin DM, Whelan SL, Ferlay J, Teppo L, Thomas DB: Cancer Incidence In Five Continents. IARC Sci Publ. 2002, 155: 258-269.Google Scholar
- Yamashita Y, Fujisawa T, Kimura A, Kato H: Epidemiology of Helicobacter pylori infection in children: a serologic study of the Kyushu region in Japan. Pediatr Int. 2001, 43: 4-7. 10.1046/j.1442-200x.2001.01337.x.PubMedView ArticleGoogle Scholar
- Epidemiology of, and risk factors for, Helicobacter pylori infection among 3194 asymptomatic subjects in 17 populations. The EUROGAST Study Group. Gut. 1993, 34: 1672-1676.Google Scholar
- Malaty HM, El-Kasabany A, Graham DY, Miller CC, Reddy SG, Srinivasan SR, Yamaoka Y, Berenson GS: Age at acquisition of Helicobacter pylori infection: a follow-up study from infancy to adulthood. Lancet. 2002, 359: 931-935. 10.1016/S0140-6736(02)08025-X.PubMedView ArticleGoogle Scholar
- Campbell DI, Warren BF, Thomas JE, Figura N, Telford JL, Sullivan PB: The African enigma: low prevalence of gastric atrophy, high prevalence of chronic inflammation in West African adults and children. Helicobacter. 2001, 6: 263-267. 10.1046/j.1083-4389.2001.00047.x.PubMedView ArticleGoogle Scholar
- Agha A, Graham DY: Evidence-based examination of the African enigma in relation to Helicobacter pylori infection. Scand J Gastroenterol. 2005, 40: 523-529.PubMedView ArticleGoogle Scholar
- Bravo LE, van Doom LJ, Realpe JL, Correa P: Virulence-associated genotypes of Helicobacter pylori: do they explain the African enigma?. Am J Gastroenterol. 2002, 97: 2839-2842. 10.1111/j.1572-0241.2002.07031.x.PubMedView ArticleGoogle Scholar
- McFarlane G, Forman D, Sitas F, Lachlan G: A minimum estimate for the incidence of gastric cancer in Eastern Kenya. Br J Cancer. 2001, 85: 1322-1325. 10.1054/bjoc.2001.1994.PubMedPubMed CentralView ArticleGoogle Scholar
- Fox JG, Beck P, Dangler CA, Whary MT, Wang TC, Shi HN, Nagler-Anderson C: Concurrent enteric helminth infection modulates inflammation and gastric immune responses and reduces helicobacter-induced gastric atrophy. Nat Med. 2000, 6: 536-542. 10.1038/75015.PubMedView ArticleGoogle Scholar
- Selin LK, Cornberg M, Brehm MA, Kim SK, Calcagno C, Ghersi D, Puzone R, Celada F, Welsh RM: CD8 memory T cells: cross-reactivity and heterologous immunity. Semin Immunol. 2004, 16: 335-347. 10.1016/j.smim.2004.08.014.PubMedView ArticleGoogle Scholar
- Williams AE, Edwards L, Hussell T: Colonic bacterial infection abrogates eosinophilic pulmonary disease. J Infect Dis. 2006, 193: 223-230. 10.1086/498915.PubMedView ArticleGoogle Scholar
- Bamford KB, Fan X, Crowe SE, Leary JF, Gourley WK, Luthra GK, Brooks EG, Graham DY, Reyes VE, Ernst PB: Lymphocytes in the human gastric mucosa during Helicobacter pylori have a T helper cell 1 phenotype. Gastroenterology. 1998, 114: 482-492. 10.1016/S0016-5085(98)70531-1.PubMedView ArticleGoogle Scholar
- Maizels RM, Yazdanbakhsh M: Immune regulation by helminth parasites: cellular and molecular mechanisms. Nat Rev Immunol. 2003, 3: 733-744. 10.1038/nri1183.PubMedView ArticleGoogle Scholar
- Mbulaiteye SM, Pfeiffer RM, Whitby D, Brubaker GR, Shao J, Biggar RJ: Human Herpesvirus 8 Infection within Families in Rural Tanzania. J Infect Dis. 2003, 187: 1780-1785. 10.1086/374973.PubMedView ArticleGoogle Scholar
- Gold BD, Khanna B, Huang LM, Lee CY, Banatvala N: Helicobacter pylori acquisition in infancy after decline of maternal passive immunity. Pediatr Res. 1997, 41: 641-646.PubMedView ArticleGoogle Scholar
- Khanna B, Cutler A, Israel NR, Perry M, Lastovica A, Fields PI, Gold BD: Use caution with serologic testing for Helicobacter pylori infection in children. J Infect Dis. 1998, 178: 460-465.PubMedView ArticleGoogle Scholar
- Hisada M, Lee MG, Hanchard B, Owens M, Song Q, van Doorn LJ, Cutler AF, Gold BD: Characteristics of Helicobacter pylori infection in Jamaican adults with gastrointestinal symptoms. J Clin Microbiol. 2001, 39: 212-216. 10.1128/JCM.39.1.212-216.2001.PubMedPubMed CentralView ArticleGoogle Scholar
- Mawas F, Feavers IM, Corbel MJ: Serotype of Streptococcus pneumoniae capsular polysaccharide can modify the Th1/Th2 cytokine profile and IgG subclass response to pneumococal-CRM(197) conjugate vaccines in a murine model. Vaccine. 2000, 19: 1159-1166. 10.1016/S0264-410X(00)00314-5.PubMedView ArticleGoogle Scholar
- Campbell DI, Pearce MS, Parker L, Thomas JE, Sullivan PB, Dale A: Immunoglobulin G subclass responses to Helicobacter pylori vary with age in populations with different levels of risk of gastric carcinoma. Clin Diagn Lab Immunol. 2004, 11: 631-633. 10.1128/CDLI.11.3.631-633.2004.PubMedPubMed CentralGoogle Scholar
- Mitchell HM, Ally R, Wadee A, Wiseman M, Segal I: Major differences in the IgG subclass response to Helicobacter pylori in the first and third worlds. Scand J Gastroenterol. 2002, 37: 517-522. 10.1080/00365520252903044.PubMedView ArticleGoogle Scholar
- Whary MT, Sundina N, Bravo LE, Correa P, Quinones F, Caro F, Fox JG: Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev. 2005, 14: 1464-1469. 10.1158/1055-9965.EPI-05-0095.PubMedView ArticleGoogle Scholar
- Segal I, Ally R, Mitchell H: Gastric cancer in sub-Saharan Africa. Eur J Cancer Prev. 2001, 10: 479-482. 10.1097/00008469-200112000-00001.PubMedView ArticleGoogle Scholar
- Poggensee G, Krantz I, Nordin P, Mtweve S, Ahlberg B, Mosha G, Freudenthal S: A six-year follow-up of schoolchildren for urinary and intestinal schistosomiasis and soil-transmitted helminthiasis in Northern Tanzania. Acta Trop. 2005, 93: 131-140. 10.1016/j.actatropica.2004.10.003.PubMedView ArticleGoogle Scholar
- Kuipers EJ, Meijer GA: Helicobacter pylori gastritis in Africa. Eur J Gastroenterol Hepatol. 2000, 12: 601-603.PubMedView ArticleGoogle Scholar
- Brubaker G, Levin AG, Steel CM, Creasey G, Cameron HM, Linsell CA, Smith PG: Multiple cases of Burkitt's lymphoma and other neoplasms in families in the North Mara District of Tanzania. Int J Cancer. 1980, 26: 165-170.PubMedView ArticleGoogle Scholar
- Eshleman JL: A study of the relative incidence of malignant tumours seen at Shirati Hospital in Tanzania. East Afr Med J. 1966, 43: 274-283.PubMedGoogle Scholar
- Kitinya JN, Lauren PA, Jones ME, Paljarvi L: Epidemiology of intestinal and diffuse types of gastric carcinoma in the Mount Kilimanjaro area, Tanzania. Afr J Med Med Sci. 1988, 17: 89-95.PubMedGoogle Scholar
- Templeton AC, Hutt MS: Distribution of tumours in Uganda. Recent Results Cancer Res. 1973, 41: 1-22.PubMedView ArticleGoogle Scholar
- Madebo T, Lindtjorn B, Henriksen TH: High incidence of oesophagus and stomach cancers in the Bale highlands of south Ethiopia. Trans R Soc Trop Med Hyg. 1994, 88: 415-10.1016/0035-9203(94)90407-3.PubMedView ArticleGoogle Scholar
- Handzel T, Karanja DM, Addiss DG, Hightower AW, Rosen DH, Colley DG, Andove J, Slutsker L, Secor WE: Geographic distribution of schistosomiasis and soil-transmitted helminths in Western Kenya: implications for anthelminthic mass treatment. Am J Trop Med Hyg. 2003, 69: 318-323.PubMedGoogle Scholar
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